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Reduced Primary Antibody Responses in a Genetic Animal Model of Depression

From the Psychology Department (E.M.F., K.A.B.), Williams College, Williamstown, MA; Skipper Bowles Center for Alcohol Studies and Department of Psychiatry (D.H.O.), University of North Carolina at Chapel Hill, Chapel Hill, NC; and Division of Molecular Medicine (D.A.L.), Wadsworth Laboratories, New York State Department of Health, Albany, NY.

View larger version (15K): [in a new window]   Fig. 1. Specific IgM and IgG antibody responses in FSL and FRL rats after immunization with 300 µg/kg KLH. (A) Anti-KLH IgM antibodies increased significantly after immunization: F(5,125) = 20.01, p < .0001. IgM levels were significantly lower in the FSL rats than in FRL controls: F(1,25) = 13.57, p < .01. Data for the 1:10 serum dilution are shown. (B) Anti-KLH IgG antibodies increased significantly after immunization: F(5,120) = 164.50, p < .0001 (IgG response values for one FRL animal were more than 2 SDs from the mean FRL response on each day; these were excluded from statistical analyses). There were no strain differences in IgG production (F(1,24) = 0.59, p = .45), and no strain by day interaction (F(5,120) = 0.49, p = .78). Data for the 1:50 serum dilution are shown. **p < .01, *p < .05.

View larger version (15K): [in a new window]   Fig. 2. Specific IgG2a and IgG1 antibody responses in FSL and FRL rats after immunization with 300 µg/kg KLH. (A) Anti-KLH IgG2a antibodies increased significantly after immunization: F(5,125) = 159.28, p < .0001. IgG2a levels were significantly lower in the FSL rats than in FRL controls (F(1,25) = 5.41, p < .05), and a significant strain by day interaction and Tukey post hoc tests indicated that strain differences were significant 11 and 14 days after immunization (F(5,125) = 4.58, p < .001). Data for the 1:10 serum dilution are shown. (B) Anti-KLH IgG1 antibodies increased significantly after immunization: F(5,125) = 81.11, p < .0001. There were no strain differences in IgG1 production (F(1,25) = 0.09, p = .77), and no strain by day interaction (F(5,125) = 0.38, p = .85). Data for the 1:10 serum dilution are shown. *p < .05.

View larger version (49K): [in a new window]   Fig. 3. KLH-stimulated IFN- production in vitro from FSL and FRL rats immunized with KLH (300 µg/kg IP) 4 days earlier. Mean (±SEM) and individual data points are shown. Data are from an assay of undiluted supernatants. #p = .01.