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Figure 1. Experimental protocol. After systemic sensitization on days 0, 14, 21 (ovalbumin (OVA) i.p.) mice were challenged twice with OVA aerosol on days 26 and 27. Coinciding with the first challenge, the stress groups (II and IV) were exposed to sound stress for 24 hours, while nonstressed mice remained undisturbed. On days 26 and 27, groups III and IV received an IP injection of the neurokinin (NK)-1 receptor antagonist before airway challenge.

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